Reduction in the thrombogenicity of biomaterials using contrasting adsorbed proteins

by Yvonne M. Lindsay

Publisher: University College Dublin in Dublin

Written in English
Published: Pages: 48 Downloads: 896
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  • Biomedical materials.,
  • Polymers in medicine.,
  • Biocomputibility.,
  • Blood -- Coagulation.,
  • Serum alumin.,
  • Blood proteins.,
  • Anticoagulants.

Edition Notes

Statementby Yvonne M. Lindsay.
ContributionsUniversity College Dublin. Department of Biochemistry.
The Physical Object
Pagination(4), iii, 48, (9)p. :
Number of Pages48
ID Numbers
Open LibraryOL17314416M

Current state of NORel polymers using the rabbit thrombogenicity model. In vivo preclinical testing of potentially new biomaterial polymers in an extracorporeal circuit (ECC) is essential in determining its interaction with flowing blood. However, this testing poses complex interactive processes between the polymer and all components of blood.   Functionalizing biomaterials with peptides or polymers that enhance recruitment of endothelial cells (ECs) can reduce blood coagulation and thrombosis. To assess endothelialization of materials in vitro, primary ECs are generally used, although the characteristics of these cells vary among the donors and change with time in culture. Recently, primary cell lines immortalized by transduction of.   The desorption of adsorbed proteins occurred in three steps: first, by adding μl/sample of PBS for 20 min under shaking, second, by adding μl/sample of % sodium dodecyl sulfate (SDS, GE Healthcare, Italy) for 20 min while shaking, and, finally, by adding μl/sample of the first-dimensional 2D-PAGE protein sample buffer (SB. PE membranes were incubated in protein solution for 24 h at 37 °C, and then washed with PBS and DI water five times to remove the weakly-bound proteins. Quantitatively, the bicinchoninic acid (BCA) protein assay kit was used to analyze the amount of adsorbed BSA where BSA was eluted from the sample by 1 wt % sodium dodecyl sulfate (SDS).

A way to avoid or minimize the side effect that could result in drug delivery to cells with increased efficiency and performance in the health rehabilitation process is to use biocompatible and biodegradable drug carriers. These are essentially biomaterials that are metallic, ceramic, or polymeric in nature. The sources of these materials must be biological in its entire ramification. Thrombogenic reactions under multiple interactions of pharmacological agents, doses, and materials have not been well understood yet. The aim of this study was to investigate the ability to simultaneously compare thrombogenic reactions to different combinations of anticoagulants, doses, and blood-contacting materials, in a single human blood using an in vitro test method. Surface modification of sodium montmorillonite (Na+-Mt) was performed using antimicrobial agents to produce an ecofriendly nanocomposite. The adsorption performance of the nanocomposite has been evaluated for the removal of Acid Blue 25 dye (AB25) as a model organic pollutant from wastewater. Sodium montmorillonite (Na+-Mt) was modified with three different ionene compounds through ion. Stent implantation represents a major step forward since the introduction of coronary angioplasty. As indications continue to expand, better understanding of the early and late biocompatibility issues appears critical. Persisting challenges to the use of intracoronary stents include the prevention of early thrombus formation and late neointima development. Different metals and designs have.

ISO is an international standard describing the methods of testing of medical devices for interactions with blood for regulatory purpose. The complexity of blood responses to biomaterial surfaces and the variability of blood functions in different individuals and species pose difficulties in standardisation. Moreover, in vivo or >in vitro testing, as well as the clinical relevance.   The adsorbed amounts of fibrinogen did not correlate with thrombogenicity in terms of TAT formation and platelet surface accumulation in blood. The combined results suggest, hence, that noble metal chemistry has a different impact on the protein . To improve the biocompatibility of biomaterials a new surface treatment has been described based on the addition of polysiloxane containing copolymers to the base polymer resin (surface modifying additives). In an in vitro and a clinical study, we compared the thrombogenicity of polyvinyl chloride (PVC) to PVC with these surface modifying additives (SMA). Blood–material interactions have been evaluated in vitro and in vivo using various materials. The most simple evaluation is the measurement of the amount of proteins adsorbed on the material’s surface. In this evaluation, single plasma protein in buffered solution is used, although more than kinds of proteins exist in plasma.

Reduction in the thrombogenicity of biomaterials using contrasting adsorbed proteins by Yvonne M. Lindsay Download PDF EPUB FB2

The assessment of the material and—at a later stage of development—the device function “(anti‐)thrombogenicity” requires the selection of different test methods, which have to be accurate, reliable, and reproducible. This is, not least, important for realizing interstudy comparisons of the thrombogenicity of different by: 2.

Methods. To manufacture the biomaterials, amorphous hydrogenated carbon nanocoatings, 80 nm thick, were deposited on silicon and stainless steel wafers by radio frequency reactive magnetron sputtering in a high vacuum chamber, in Ar/H 2 atmosphere by varying the hydrogen content (from 5% to 20% H 2).Two types of carbon thin films were developed under different deposition conditions: type A Cited by: In Vitro Thrombogenicity Testing of Biomaterials Steffen Braune, Robert A.

Latour, Markus Reinthaler, Ulf Landmesser, Andreas Lendlein, different undesired pathophy-siological processes are described: plasma protein adsorp- concentrate on the interaction of blood plasma proteins and platelets with polymer-based by: 2.

In Vitro Thrombogenicity Testing Reduction in the thrombogenicity of biomaterials using contrasting adsorbed proteins book resolving different proteins of various molecular masses. The and visualizing adsorbed proteins after contacting material sur.

Comparative thrombogenicity of biomaterials used in the cardiovascular prostheses were quantified with Indium labeled ovine platelets and Iodine labeled thrombin (ITH). Ovine platelets were labeled with In tropolone.

Thrombin was labeled by iodogen-transfer technique with I iodide at a high specific activity. National Heart, Lung and Blood Institute (U.S.) NI of H, Guidelines for Physicochemical Characterization of Biomaterials (U.S.

Department of Health and Human Services, Public Health Service, National Institutes of Health, Bethesda, MD, ). In contrast to the complexity of modifying the surface with active biomolecules or cells, the relatively simpler concept of reducing protein adsorption, the presumed first step in device thrombosis, through surface modification has received considerable attention [49,83].Hydrophilic [84], hydrophobic [14], and zwitter-ionic [85,86] surfaces.

Due to the contact between biomaterials and the blood, testing the thrombogenicity and hemocompatibility evolved as additional mandatory parts of in vitro investigations[ 1. Here, the status of in vitro thrombogenicity testing methods for biomaterials is reviewed, particularly taking in view the preparation of test materials and references, the selection and characterization of donors and blood samples, the prerequisites for reproducible approaches and applied test systems.

Reduction of metal ion release, platelet activation, and thrombogenicity, Thromb Res 99 (), – [39] K.H. Grotemeyer, The platelet-reactivity-test- a useful “by-product” of the. The amount of plasma protein adsorbed on a phospholipid polymer having a 2-methacryloyloxyethyl phosphorylcholine (MPC) moiety was reduced compared to the amount of protein adsorbed.

The thrombogenicity of a material surface is described as one of the major reasons for the failure of blood-contacting medical devices [51].Consequently and as described in the ISO (Selection of Tests for Interactions of Blood), the examination of material-induced thrombosis (thrombogenicity) is one of the key requirements in the evaluation of new biomaterials (Fig.

Depending on the composition of the adsorbed protein layer, the conformation of the proteins and the shear rate of the local blood flow, passing platelets can adhere (according to the shear rates different plasma proteins mediate the platelet adhesion via different platelet membrane receptors).

This initiates abruptly an activation process so. Quantification of adsorbed protein on the different monolayers was performed using I-labelled α-thrombin (Haematologic Technologies Inc., 1 mg ml−1 in 50 mM sodium phosphate, mM sodium chloride, pH ; U mg−1) and human serum albumin (HSA; Sigma–Aldrich, Ref.

A) were labelled using the iodogen method [ Buddy D. Ratner, Thomas A. Horbett, in Biomaterials Science (Third Edition), Platelet Adhesion Measured In vitro (Cao et al., ). Platelet adhesion measured under in vitro conditions is often used as an indicator of surface thrombogenicity, because.

In contrast, desorption of two proteins from the grafted PEG layer generated by a UV oxidation method resulted in near-zero adsorbed amount. on endothelial cell thrombogenicity. Biomaterials. Cardiovascular biomaterials (CB) dominate the category of biomaterials based on the demand and investments in this field.

This review article classifies the CB into three major classes, namely, metals, polymers, and biological materials and collates the information about the CB.

Blood compatibility is one of the major criteria which limit the use of biomaterials for cardiovascular application. The reduction of nonspecific protein adsorption plays a key role in improving the compat ibility and efficiency of biomaterials.

Modifying surfaces to achieve this goal is currently a complicated process, while the primary approach used is through chemical modification of. Adsorbed proteins can form a surface monolayer with a thickness of 2–10 nm, and the concentrations of proteins on the surface can be ‐fold higher than those in plasma 2.

Surface adsorption is a reversible process, and the composition of absorbed proteins changes over time, a phenomenon known as the Vroman effect.

The results indicated that the in vitro thrombogenicity test method was capable of assessing differences in platelet factor 4 and β‐thromboglobulin increases among different combinations of the two materials, two anticoagulants, and two ACTs.

Higher amounts of total plasma proteins were absorbed on PVC tubes than on PMEA‐coated tubes when. Research on nanoparticles obtained on biological supports is a topic of growing interest in nanoscience, especially regarding catalytic applications. Silver nanoparticles (AgNPs) have been studied due to their low toxicity, but they tend to aggregation, oxidation, and low stability.

In this work, we synthesized and characterized AgNPs supported on S-layer proteins (SLPs) as bidimensional. An essential criteria for any vascular graft is hemocompatibility to prevent thrombosis, a primary cause of graft failure [].As reviewed previously, the process of biomaterial-induced thrombosis is mediated by the binding of platelets to the rapidly adsorbed proteins on the biomaterial or tissue-engineered surface [].Thrombosis has been particularly well-studied in vitro.

(BioLinx). The use of different polymers (in addition to changes in stent platforms) contributed to a reduction in late ST rates relative to earlier-generation DESs,12 Despite these improvements, the association of durable polymers with potentially harmful effects lingered, and the assumption that BMS had a greater biocompatibility.

A preliminary consideration of the blood interactions of the CF 3-functionalized polyester was evaluated by measuring the amount of the adsorbed albumin and fibrinogen from human blood plasma. The fluorinated polyester adsorbed and retained higher amounts of albumin and fibrinogen with a higher albumin/fibrinogen ratio as compared to poly.

Wagner, M.S., T.A. Horbett, and D.G. Castner, Characterization of the structure of binary and ternary adsorbed protein films using electron spectroscopy for chemical analysis, time-of-flight secondary ion mass spectrometry, and radiolabeling. Langmuir, 19(5)– Google Scholar.

This work focuses on understanding the thrombogenicity of the polymer by examining mechanistic interactions with proteins, human platelets, and human monocytes of a number of polymers used in drug eluting stent coatings, in vitro.

The importance for blood interactions of adsorbed albumin and the retention of albumin was suggested by the data.

In previous studies, we have developed a methodology to study the possibility of thrombus formation during material–protein interactions, the optical properties of the adsorbed plasma proteins, and their adsorption mechanisms,14 Ex-situ spectroscopic ellipsometry measurements in the energy region of – eV of human albumin and.

Hence, it is the adsorbed proteins, rather than the surface itself, to which cells initially respond. Diverse studies using a range of materials have demonstrated the pivotal role of extracellular adhesion proteins—fibronectin and vitronectin in particular—in cell adhesion, morphology, and migration.

role of adsorbed proteins in cell interactions with solid surfaces Mechanism of the Initial Attachment of Human Vein Endothelial Cells onto Polystyrene-Based Culture Surfaces and Surfaces Prepared by Radiofrequency Plasmas.

Abstract. The development of non-thrombogenic materials and surface treatments are ongoing needs in the biomaterials area. Also, the need for improved test procedures for evaluating new materials in terms of thrombogenicity continues to be a requirement. 1,2 In the current context, results of surface treatments for improved non-thrombogenicity along with a description of the test method.

The changes in the secondary structure of surface-adsorbed proteins were evaluated using FTIR-ATR spectroscopy. For this purpose, second-derivative and curve-fitting (CF) procedures were performed. In the fitting procedure, the amide I band was treated to give a linear baseline between and cm −1.

Fitting was performed assuming.Since adsorbed protein forms a viscoelastic film, the Sauerbrey relation overestimates the real mass of adsorbed proteins onto the surface. The Voigt viscoelastic modeling in QTools (Q tools, Q-sense AB, Sweden) was thus applied to estimate the adsorbed mass per surface unit by considering both frequency (Δ f) and dissipation (Δ D) shifts.Adsorption to Biomaterials from Protein Mixtures Thomas A.

Horbett Chap DOI: /bkch Publication Date (Print): J