Reduction in the thrombogenicity of biomaterials using contrasting adsorbed proteins

Reduction in the thrombogenicity of biomaterials using contrasting adsorbed proteins by Yvonne M. Lindsay Download PDF EPUB FB2

The assessment of the material and—at a later stage of development—the device function “(anti‐)thrombogenicity” requires the selection of different test methods, which have to be accurate, reliable, and reproducible. This is, not least, important for realizing interstudy comparisons of the thrombogenicity of different by: 2.

Methods. To manufacture the biomaterials, amorphous hydrogenated carbon nanocoatings, 80 nm thick, were deposited on silicon and stainless steel wafers by radio frequency reactive magnetron sputtering in a high vacuum chamber, in Ar/H 2 atmosphere by varying the hydrogen content (from 5% to 20% H 2).Two types of carbon thin films were developed under different deposition conditions: type A Cited by: In Vitro Thrombogenicity Testing of Biomaterials Steffen Braune, Robert A.

Latour, Markus Reinthaler, Ulf Landmesser, Andreas Lendlein, different undesired pathophy-siological processes are described: plasma protein adsorp- concentrate on the interaction of blood plasma proteins and platelets with polymer-based by: 2.

In Vitro Thrombogenicity Testing Reduction in the thrombogenicity of biomaterials using contrasting adsorbed proteins book resolving different proteins of various molecular masses. The and visualizing adsorbed proteins after contacting material sur.

Comparative thrombogenicity of biomaterials used in the cardiovascular prostheses were quantified with Indium labeled ovine platelets and Iodine labeled thrombin (ITH). Ovine platelets were labeled with In tropolone.

Thrombin was labeled by iodogen-transfer technique with I iodide at a high specific activity. National Heart, Lung and Blood Institute (U.S.) NI of H, Guidelines for Physicochemical Characterization of Biomaterials (U.S.

Department of Health and Human Services, Public Health Service, National Institutes of Health, Bethesda, MD, ). In contrast to the complexity of modifying the surface with active biomolecules or cells, the relatively simpler concept of reducing protein adsorption, the presumed first step in device thrombosis, through surface modification has received considerable attention [49,83].Hydrophilic [84], hydrophobic [14], and zwitter-ionic [85,86] surfaces.

Due to the contact between biomaterials and the blood, testing the thrombogenicity and hemocompatibility evolved as additional mandatory parts of in vitro investigations[ 1. Here, the status of in vitro thrombogenicity testing methods for biomaterials is reviewed, particularly taking in view the preparation of test materials and references, the selection and characterization of donors and blood samples, the prerequisites for reproducible approaches and applied test systems.

Reduction of metal ion release, platelet activation, and thrombogenicity, Thromb Res 99 (), – [39] K.H. Grotemeyer, The platelet-reactivity-test- a useful “by-product” of the. The amount of plasma protein adsorbed on a phospholipid polymer having a 2-methacryloyloxyethyl phosphorylcholine (MPC) moiety was reduced compared to the amount of protein adsorbed.

The thrombogenicity of a material surface is described as one of the major reasons for the failure of blood-contacting medical devices [51].Consequently and as described in the ISO (Selection of Tests for Interactions of Blood), the examination of material-induced thrombosis (thrombogenicity) is one of the key requirements in the evaluation of new biomaterials (Fig.

Depending on the composition of the adsorbed protein layer, the conformation of the proteins and the shear rate of the local blood flow, passing platelets can adhere (according to the shear rates different plasma proteins mediate the platelet adhesion via different platelet membrane receptors).

This initiates abruptly an activation process so. Quantification of adsorbed protein on the different monolayers was performed using I-labelled α-thrombin (Haematologic Technologies Inc., 1 mg ml−1 in 50 mM sodium phosphate, mM sodium chloride, pH ; U mg−1) and human serum albumin (HSA; Sigma–Aldrich, Ref.

A) were labelled using the iodogen method [ Buddy D. Ratner, Thomas A. Horbett, in Biomaterials Science (Third Edition), Platelet Adhesion Measured In vitro (Cao et al., ). Platelet adhesion measured under in vitro conditions is often used as an indicator of surface thrombogenicity, because.

In contrast, desorption of two proteins from the grafted PEG layer generated by a UV oxidation method resulted in near-zero adsorbed amount. on endothelial cell thrombogenicity. Biomaterials. Cardiovascular biomaterials (CB) dominate the category of biomaterials based on the demand and investments in this field.

This review article classifies the CB into three major classes, namely, metals, polymers, and biological materials and collates the information about the CB.

Blood compatibility is one of the major criteria which limit the use of biomaterials for cardiovascular application. The reduction of nonspecific protein adsorption plays a key role in improving the compat ibility and efficiency of biomaterials.

Modifying surfaces to achieve this goal is currently a complicated process, while the primary approach used is through chemical modification of. Adsorbed proteins can form a surface monolayer with a thickness of 2–10 nm, and the concentrations of proteins on the surface can be ‐fold higher than those in plasma 2.

Surface adsorption is a reversible process, and the composition of absorbed proteins changes over time, a phenomenon known as the Vroman effect.

The results indicated that the in vitro thrombogenicity test method was capable of assessing differences in platelet factor 4 and β‐thromboglobulin increases among different combinations of the two materials, two anticoagulants, and two ACTs.

Higher amounts of total plasma proteins were absorbed on PVC tubes than on PMEA‐coated tubes when. Research on nanoparticles obtained on biological supports is a topic of growing interest in nanoscience, especially regarding catalytic applications. Silver nanoparticles (AgNPs) have been studied due to their low toxicity, but they tend to aggregation, oxidation, and low stability.

In this work, we synthesized and characterized AgNPs supported on S-layer proteins (SLPs) as bidimensional. An essential criteria for any vascular graft is hemocompatibility to prevent thrombosis, a primary cause of graft failure [].As reviewed previously, the process of biomaterial-induced thrombosis is mediated by the binding of platelets to the rapidly adsorbed proteins on the biomaterial or tissue-engineered surface [].Thrombosis has been particularly well-studied in vitro.

(BioLinx). The use of different polymers (in addition to changes in stent platforms) contributed to a reduction in late ST rates relative to earlier-generation DESs,12 Despite these improvements, the association of durable polymers with potentially harmful effects lingered, and the assumption that BMS had a greater biocompatibility.

A preliminary consideration of the blood interactions of the CF 3-functionalized polyester was evaluated by measuring the amount of the adsorbed albumin and fibrinogen from human blood plasma. The fluorinated polyester adsorbed and retained higher amounts of albumin and fibrinogen with a higher albumin/fibrinogen ratio as compared to poly.

Wagner, M.S., T.A. Horbett, and D.G. Castner, Characterization of the structure of binary and ternary adsorbed protein films using electron spectroscopy for chemical analysis, time-of-flight secondary ion mass spectrometry, and radiolabeling. Langmuir, 19(5)– Google Scholar.

This work focuses on understanding the thrombogenicity of the polymer by examining mechanistic interactions with proteins, human platelets, and human monocytes of a number of polymers used in drug eluting stent coatings, in vitro.

The importance for blood interactions of adsorbed albumin and the retention of albumin was suggested by the data.

In previous studies, we have developed a methodology to study the possibility of thrombus formation during material–protein interactions, the optical properties of the adsorbed plasma proteins, and their adsorption mechanisms,14 Ex-situ spectroscopic ellipsometry measurements in the energy region of – eV of human albumin and.

Hence, it is the adsorbed proteins, rather than the surface itself, to which cells initially respond. Diverse studies using a range of materials have demonstrated the pivotal role of extracellular adhesion proteins—fibronectin and vitronectin in particular—in cell adhesion, morphology, and migration.

role of adsorbed proteins in cell interactions with solid surfaces Mechanism of the Initial Attachment of Human Vein Endothelial Cells onto Polystyrene-Based Culture Surfaces and Surfaces Prepared by Radiofrequency Plasmas.

Abstract. The development of non-thrombogenic materials and surface treatments are ongoing needs in the biomaterials area. Also, the need for improved test procedures for evaluating new materials in terms of thrombogenicity continues to be a requirement. 1,2 In the current context, results of surface treatments for improved non-thrombogenicity along with a description of the test method.

The changes in the secondary structure of surface-adsorbed proteins were evaluated using FTIR-ATR spectroscopy. For this purpose, second-derivative and curve-fitting (CF) procedures were performed. In the fitting procedure, the amide I band was treated to give a linear baseline between and cm −1.

Fitting was performed assuming.Since adsorbed protein forms a viscoelastic film, the Sauerbrey relation overestimates the real mass of adsorbed proteins onto the surface. The Voigt viscoelastic modeling in QTools (Q tools, Q-sense AB, Sweden) was thus applied to estimate the adsorbed mass per surface unit by considering both frequency (Δ f) and dissipation (Δ D) shifts.Adsorption to Biomaterials from Protein Mixtures Thomas A.

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